Application of Whole Mouse Embryo Culture System on Stem Cell Research

P-126: Stem Cell Factor Increases Blastocyst Formation in Mouse Two-Cell Embryo Culture

Background: It is demonstrated that c-Kit( receptor of stem cell factor) mRNA is expressed in late 2-cell stage to the expanded and hatched blastocyst and the stem cell factor (SCF) transcript is detected in the oviduct and uterus. The aim of this study was to investigate the effect of different doses of SCF on mouse 2-cell embryo development in vitro. Materials and Methods: 4-6 weeks old femal...

Effect of Two Polarized Culture System Prepared From Human Female Genital Tract on Mouse Embryo Development

Purpose: To compare effects of polarized epithelial monolayer prepared from human oviduct and uterus on mouse one and two-cell embryo development. Materials and Methods: Human oviduct and endometrial tissue was obtained from patients who had undergone total hysterectomy. The epithelial cells were isolated from tissue and cultured on extracellular matrix (ECM) Gel coated Millipore filter insert...

P-92: Retinoic Acid and STO Co-Culture System Enhance Mouse Embryonic Stem Cell

[Experimental study on cleft lip of CL/Fr mouse using whole embryo culture system].

The incidence of cleft lip in CL/Fr mouse is 18-2500. The purpose of this study is to clarify the cause of cleft lip in CL/Fr mouse using whole embryo culture system which is a useful technique in studying the development of mammalian embryos in vitro. We examined the incidence of cleft lip in CL/Fr and F, mouse embryo (male CL/Fr x female C57BL/6) in vitro. The embryos were explanted on day 10...

Effect of LH Treated Ovine Oviductal Epithelial Cell Co-Culture System on Murine Pre-Embryo Development

Background This study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (LH) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. MaterialsAndMethods Monolayers for co-culture were prepared from ovine oviduct epithelial cells (OOEC) in DMEM/F12 medium a...